CytoCell C-MET (MET) Amplification FISH Probe

Product summary

Technology FISH
Application Solid tumour
Areas of interest Breast cancer, Colorectal cancer, Pancreatic cancer, Thyroid cancer, Ovarian cancer, Lung cancer, Sarcoma
Region 7q31.2 / 7p11.1-q11.1
Label
Product Code LPS 004 (10 tests)
LPS 004-S (5 tests)
Regulatory Status In vitro diagnostic. This product is intended to be used on formalin-fixed paraffin-embedded (FFPE) tissues. Disease information supported by the literature and is not a reflection of the intended purpose of this product.

Documentation

Overview

Probe specification

C-MET, 7q31.2, Red
D7Z1, 7p11.1-q11.1, Green

The C-MET Amplification probe consists of a 278kb red probe spanning the C-MET (MET) gene. The centromeric probe in green acts as a control for chromosome 7.

Probe information

The C-MET (MET - Mesenchymal-Epithelial Transition factor) encodes a transmembrane tyrosine kinase¹, which is an oncoprotein.

The MET gene regulates both cell motility and cell growth², so normal C-MET expression allows stem cells and progenitor cells to grow invasively. This invasive growth is required to generate new tissues in an embryo, or in an adult in regenerating tissue, such as the liver, or during wound repair³.

MET has been shown to be overexpressed in many tumours including ovarian⁴, breast⁵, lung⁶, thyroid⁷, stomach⁸, pancreatic^9,10 and colon^11,12. This overexpression correlates with a poor prognosis^4,13,14. In breast cancers, MET was only shown to be co-expressed with HER2 (ERBB2) in 50% of patients, indicating that it has a significant impact on tumour aggressiveness independently of HER2¹³.

Fluorescence in situ hybridisation (FISH) microscope image of the CytoCell C-MET (MET) Amplification probe.

What our customers say

The quality and reproducibility of results using the CytoCell kit has been vital in accurately detecting co-deletions in our glioma investigations. We now have a cost-effective test that we can rely on that is also easy to use and interpret. We've been consistently impressed with this kit - not to mention the support offered by OGT's customer service, and have completely transitioned over to CytoCell probes.

Gavin Cuthbert, FRCPath

Head of Cancer Cytogenetics, Northern Genetics Service, Newcastle, UK

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References

Dean M et al., Nature 1985;318:385-8
Gherardi E, Stoker M, Cancer Cells 1991;3(6):227-32
Boccaccio C, Comoglio PM, Nat Rev Cancer 2006;6(8):637-45
Sawada K et al., Cancer Res 2007;67(4):1670-9
Carracedo A et al., Breast Cancer Res 2009;11(2):402
Zucali PA et al., Ann Oncol 2008;19(9):1605-12
Di Renzo MF et al., Oncogene 1992;7:2549-53
Kuniyasu H et al., Int J Cancer 1993;55(1):72-5
Ebert M et al., Cancer Res 1994;54:5775-8
Di Renzo MF et al., Cancer Res 1995;55:1129-38
Liu C et al., Oncogene 1992;7(1):181-5
Di Renzo MF et al., Clin Cancer Res 1995;1:147-54
Miyamoto et al., Br J Cancer. 2011 Jun 28;105(1):131-8
Lengyel E et al., Int J Canc 2005;113:678-82

FFPE tissue preparation and FISH protocol

Select a protocol step to view:

Heat pretreatment

Heat 50ml Tissue Pretreatment Solution (Reagent 1) in a porcelain wash jar or coplin jar immersed in a waterbath until it is either boiling or 98 - 100°C.
Boil slides for 30 minutes (Note: different incubation times may be required depending on tissue fixation. A 30-minute incubation is a recommended starting point).
Wash in PBS or dH₂O at room temperature (RT) for 2 x 3 minutes.