CytoCell IGH/BCL2 Translocation FISH Probe (Solid Tumour)

Product summary

Technology FISH
Application Solid tumour
Areas of interest Lymphoma
Region 14q32.33
18q21.33-q22.1
Label
Product Code LPS 033 (10 tests)
LPS 033-S (5 tests)
Regulatory Status In vitro diagnostic. This product is intended to be used on Carnoy’s solution (3:1 methanol/acetic acid) fixed haematological samples, or formalin-fixed paraffin-embedded (FFPE) tissues. Disease information supported by the literature and is not a reflection of the intended purpose of this product.

Documentation

Overview

Probe specification

IGH, 14q32.33, Green
BCL2, 18q21.33-q22.1, Red

The IGH/BCL2 product consists of probes, labelled in green, covering the Constant, J, D and Variable segments of the IGH gene, and four probes labelled in red, positioned centromeric and telomeric to the BCL2 gene.

Probe information

The IGH/BCL2 fusion gene is brought about by a t(14;18)(q32;q21) translocation and is found in over 88% of follicular lymphoma cases^1,2.

The translocation is thought to be brought about by an error in the joining function of the IGH gene, mediated by the recent observation that both IGH and BCL2 are arranged next to each other in 3D space in the normal B lymphocytes². The translocation breakpoint at the end of the Joining (J) segment, and the subsequent fusion of the BCL2 gene to this region, results in the BCL2 gene coming under the regulatory control of those processes normally involved in maintenance of IGH gene activity³.

The BCL2 gene itself has been shown to be involved in the regulation of apoptosis⁴.

Fluorescence in situ hybridisation (FISH) microscope image of the CytoCell IGH/BCL2 Translocation, Dual Fusion probe.

What our customers say

In our hands, CytoCell FISH probes, including the ROS1 Proximal and ROS1 Distal probes, have proven to be of the highest quality with bright, easy to interpret signals, thus providing confidence in our results. OGT's customer support is outstanding, as their staff are extremely knowledgeable and truly care about their customers and their customers’ needs.

Jennie Thurston

Director of Cytogenetics, Carolinas Pathology Group, USA

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FFPE tissue preparation and FISH protocol

Select a protocol step to view:

Heat pretreatment

Heat 50ml Tissue Pretreatment Solution (Reagent 1) in a porcelain wash jar or coplin jar immersed in a waterbath until it is either boiling or 98 - 100°C.
Boil slides for 30 minutes (Note: different incubation times may be required depending on tissue fixation. A 30-minute incubation is a recommended starting point).
Wash in PBS or dH₂O at room temperature (RT) for 2 x 3 minutes.