CytoCell ROS1 Plus Breakapart FISH Probe

Product summary

Technology FISH
Application Solid tumour
Areas of interest Lung cancer
Region 6q22.1
Label
Product Code LPS 046 (10 tests)
LPS 046-S (5 tests)
Regulatory Status In vitro diagnostic. This product is intended to be used on formalin-fixed paraffin-embedded (FFPE) tissues. Disease information supported by the literature and is not a reflection of the intended purpose of this product.

Documentation

Overview

Probe specification

ROS1 Plus, 6q22.1, Green
ROS1 Plus, 6q22.1, Red

The ROS1 Plus probe consists of 3 probes (175kb, 123kb, 171kb), labelled in green, situated proximal to the ROS1 gene and including markers D6S2428 and SHGC-149552 and a red probe (206kb) covering the distal part of the ROS1 gene and a region up to RH78348.

Probe information

The ROS1 (ROS proto-oncogene 1, receptor tyrosine kinase) gene at 6q22 is an ALK (anaplastic lymphoma receptor tyrosine kinase) gene paralogue which encodes a type I integral membrane protein with tyrosine kinase activity¹.

ROS1 rearrangements define a molecular subset of non-small cell lung cancer (NSCLC) and are seen in approximately 2% of patients with NSCLC². A number of partner genes have been identified, including SLC34A2, CD74 and SDC4³. It has been shown that these ROS1 fusions activate the pSTAT3, PI3K/AKT/mTOR and SHP-2 phosphatase pathways^4,5.

NSCLC patients with ROS1 rearrangements have been shown to respond to treatment with ALK/MET tyrosine kinase inhibitors, such as crizotinib⁶.

ROS1 rearrangements with the GOPC (golgi associated PDZ and coiled-coil motif containing) gene fusion partner were originally reported in glioblastoma, but have now also been detected on cholangiocarcinoma, ovarian cancer and NSCLC patient samples^7-9.

The ROS1 Plus design covers the ROS1 region and the region deleted in ROS1-GOPC fusions.

Fluorescence in situ hybridisation (FISH) microscope image of the CytoCell ROS1 Plus Breakapart probe.

What our customers say

In our hands, CytoCell FISH probes, including the ROS1 Proximal and ROS1 Distal probes, have proven to be of the highest quality with bright, easy to interpret signals, thus providing confidence in our results. OGT's customer support is outstanding, as their staff are extremely knowledgeable and truly care about their customers and their customers’ needs.

Jennie Thurston

Director of Cytogenetics, Carolinas Pathology Group, USA

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References

Matsushime H et al., Mol Cell Biol 1986;6:3000-4
Bergethon K et al., J Clin Oncol 2012;30(8):863-70
Davies KD et al., Cancer Res 2012;72(8):1538-7445
Birchmeier C et al., Proc Natl Acad Sci 1987;84:9270-9274
Ou SH et al., Expert Rev Anticancer Ther 2012;12(4):447-56
Shaw, A.T. et al., 2014. 371(21), pp.1963–71
Gu TL et al., PLoS One 2011;6: e15640
Charest A et al., Genes Chromosomes Cancer 2003;37:58-71
Davies KD & Doebele RC, 2013. Clinical cancer 19(15), 4040–4045

FFPE tissue preparation and FISH protocol

Select a protocol step to view:

Heat pretreatment

Heat 50ml Tissue Pretreatment Solution (Reagent 1) in a porcelain wash jar or coplin jar immersed in a waterbath until it is either boiling or 98 - 100°C.
Boil slides for 30 minutes (Note: different incubation times may be required depending on tissue fixation. A 30-minute incubation is a recommended starting point).
Wash in PBS or dH₂O at room temperature (RT) for 2 x 3 minutes.