Tips include advice on preparing the sequencing pool and how to avoid the inaccurate demultiplexing of your samples.
Prepare Sequencing Pool: Use pooling worksheet to sequence equimolar amounts of libraries for sequencing based on laboratory recommendation.
Kicki Bergefall
FAS Manager - Europe
Prepare sample sheet: make sure the assay is single-index, paired end sequencing and use the proper setting from the sequencer to verify sample sheet parameters. If the sample sheet is incorrectly prepared, demultiplexing of your samples will not be carried out accurately. This would lead to difficulties in data analysis.
Serena Pace
NGS Field Application Scientist, North America
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