Tips include why you should work swiftly and why you should not discard the beads during the final wash steps.
Ensure the temperature of wash beads/buffers do not drop below the recommended temperature in the handbook.
Huiyan Jin
NGS Field Application Scientist, North America
Work swiftly to ensure the temperature does not drop between wash steps.
Pete Gray
Array and NGS Field Application Specialist, Europe
Captured DNA remains on the beads and will proceed into the Post-Capture PCR. DO NOT discard the beads during the final wash steps, as you would lose your enriched DNA. The Streptavidin beads do not affect the efficiency of the post-capture PCR.
Kicki Bergefall
FAS Manager - Europe
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