CytoCell Alpha Satellite 12 Plus for CLL FISH Probe

Product summary

Technology FISH
Application Haematology
Areas of interest CLL
Region 12p11.1-q11.1
Label
Product Code LPH 069 (10 tests)
LPH 069-S (5 tests)
Regulatory Status In vitro diagnostic. This product is intended to be used on Carnoy’s solution (3:1 methanol/acetic acid) fixed haematological samples. Disease information supported by the literature and is not a reflection of the intended purpose of this product.

Documentation

Overview

Probe specification

D12Z3, 12p11.1-q11.1, Red

The Alpha Satellite 12 Plus Probe is a repeat sequence probe, labelled in red, which recognises the centromeric repeat sequence D12Z3.

Probe information

Trisomy 12 is a recurrent abnormality in CLL, seen in 20% of cases¹ that often appears as the unique cytogenetic aberration (40-60% of cases with trisomy 12)². Patients with trisomy 12 are classified as low-risk in the absence of any other genetic lesions³.

This product has been optimised for overnight hybridisation and is also available as part of the CLL Plus Screening Panel (LPH 087).

Fluorescence in situ hybridisation (FISH) microscope image of the CytoCell Alpha Satellite 12 Plus for CLL probe.

What our customers say

Not only do CytoCell offer an extensive range of high-quality FISH probes, the customer support is also excellent — providing fast access to all the probes I need. The probes are highly consistent with bright signals allowing easy scoring of results.

Dr Eric Crawford

Senior Director, Genetics Associates Inc., USA

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Recommended protocol for CytoCell haematology FISH

Select a protocol step to view:

Sample and slide preparation

Spot the cell sample onto a glass microscope slide. Allow to dry.
Immerse the slide in 2x Saline Sodium Citrate (SSC) for 2 minutes at room temperature (RT) without agitation.
Dehydrate in an ethanol series (70%, 85% and 100%), each for 2 minutes at RT.
Allow to dry.