CytoCell EML4 Breakapart FISH Probe

Product summary

Technology FISH
Application Solid tumour
Areas of interest Lung cancer
Region 2p21
Label
Product Code LPS 020 (10 tests)
LPS 020-S (5 tests)
Regulatory Status In vitro diagnostic. This product is intended to be used on formalin-fixed paraffin-embedded (FFPE) tissues. Disease information supported by the literature and is not a reflection of the intended purpose of this product.

Documentation

Overview

Probe specification

EML4, 2p21, Red
EML4, 2p21, Green

The EML4 Breakapart probe consists of a green 406kb probe and a red 353kb probe, which are positioned on each side of the EML4 gene.

Probe information

The protein encoded by the echinoderm microtubule associated protein-like 4 (EML4) gene is involved in microtubule formation and stabilisation¹.

A novel gene fusion of EML4 and Anaplastic Lymphoma Kinase (ALK) was recently identified in Non-Small Cell Lung Cancer (NSCLC).

The EML4-ALK fusion was shown to result from an inversion within chromosome 2p, inv(2)(p21p23), and was detected in approximately 7% of NSCLC cases^2,3.

Fluorescence in situ hybridisation (FISH) microscope image of the CytoCell EML4 Breakapart probe.

What our customers say

In our hands, CytoCell FISH probes, including the ROS1 Proximal and ROS1 Distal probes, have proven to be of the highest quality with bright, easy to interpret signals, thus providing confidence in our results. OGT's customer support is outstanding, as their staff are extremely knowledgeable and truly care about their customers and their customers’ needs.

Jennie Thurston

Director of Cytogenetics, Carolinas Pathology Group, USA

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FFPE tissue preparation and FISH protocol

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Heat pretreatment

Heat 50ml Tissue Pretreatment Solution (Reagent 1) in a porcelain wash jar or coplin jar immersed in a waterbath until it is either boiling or 98 - 100°C.
Boil slides for 30 minutes (Note: different incubation times may be required depending on tissue fixation. A 30-minute incubation is a recommended starting point).
Wash in PBS or dH₂O at room temperature (RT) for 2 x 3 minutes.