CytoCell EWSR1/ERG Translocation, Dual Fusion FISH Probe

Product summary

Technology FISH
Application Solid tumour
Areas of interest Sarcoma
Region 22q12.1-q12.2
21q22.13-q22.2
Label
Product Code LPS 008 (10 tests)
LPS 008-S (5 tests)
Regulatory Status In vitro diagnostic. This product is intended to be used on formalin-fixed paraffin-embedded (FFPE) tissues. Disease information supported by the literature and is not a reflection of the intended purpose of this product.

Documentation

Overview

Probe specification

EWSR1, 22q12, Red
ERG, 21q22, Green

The EWSR1/ERG Translocation probe consists of green probes (215kb, 154kb) flanking the breakpoint region at the ERG gene locus and red probes (139kb, 151kb) flanking the breakpoint region at the EWSR1 locus.

Probe information

Ewing's sarcoma (EWS) is the second most frequent primary bone cancer in patients under 20 years of age¹. It is mainly characterised by a translocation between the two genes - EWSR1 (Ewing's Sarcoma Region 1) and FLI1 (Friend Leukaemia virus Integration 1) - in the t(11;22)(q24;q12) translocation, found in 83% of such tumours². 10% of the remaining cases have a variant translocation involving EWSR1 and ERG, t(21;22)(q22;q12), and less than 1% carry one of the 7p22 (ETV1), 17q12 (E1AF) or 2q36 (FEV) translocations with EWSR13.

EWSR1 is also involved in translocations in desmoplasmic small round cell tumours, a subset of angiomatoid fibrous histocytomas, and possibly in myxoid liposarcoma^4,5.

Fluorescence in situ hybridisation (FISH) microscope image of the CytoCell EWSR1/ERG Translocation, Dual Fusion probe.

What our customers say

The quality and reproducibility of results using the CytoCell kit has been vital in accurately detecting co-deletions in our glioma investigations. We now have a cost-effective test that we can rely on that is also easy to use and interpret. We've been consistently impressed with this kit - not to mention the support offered by OGT's customer service, and have completely transitioned over to CytoCell probes.

Gavin Cuthbert, FRCPath

Head of Cancer Cytogenetics, Northern Genetics Service, Newcastle, UK

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FFPE tissue preparation and FISH protocol

Select a protocol step to view:

Heat pretreatment

Heat 50ml Tissue Pretreatment Solution (Reagent 1) in a porcelain wash jar or coplin jar immersed in a waterbath until it is either boiling or 98 - 100°C.
Boil slides for 30 minutes (Note: different incubation times may be required depending on tissue fixation. A 30-minute incubation is a recommended starting point).
Wash in PBS or dH₂O at room temperature (RT) for 2 x 3 minutes.