CytoCell IGH/MYEOV Plus Translocation FISH Probe

Product summary

Technology FISH
Application Haematology
Areas of interest Lymphoma, MM
Region 11q13.3
14q32.33
Label
Product Code LPH 078 (10 tests)
LPH 078-S (5 tests)
Regulatory Status In vitro diagnostic. This product is intended to be used on Carnoy’s solution (3:1 methanol/acetic acid) fixed haematological samples. Disease information supported by the literature and is not a reflection of the intended purpose of this product.

Documentation

Chromomaps

14q32.33 (IGH)

Fluorescence in situ hybridisation (FISH) chromosome map showing a probe targeting the IGH gene.

11q13.3 (MYEOV)

Fluorescence in situ hybridisation (FISH) chromosome map showing a probe targeting the MYEOV gene.

Overview

Probe specification

IGH, 14q32.33, Green
MYEOV, 11q13.3, Red

The IGH/MYEOV Plus product consists of probes, labelled in green, proximal to the Constant, and within the Variable segment of the IGH region and MYEOV probes, labelled in red. The MYEOV probe mix contains a 155kb probe centromeric to the MYEOV gene, which includes the TPCN2 gene and a second probe, telomeric to the MYEOV gene, covering the 162kb region including the CCND1 and ORAOV1 genes.

Probe information

The MYEOV (myeloma overexpressed) gene is located at 11q13.3 and IGH (immunoglobulin heavy locus) at 14q32.33.

Approximately 50-60% of multiple myeloma (MM) cases are associated with translocations involving IGH and one of several partners including CCND1, NSD2 (WHSC1) and FGFR3, CCND3, MAF or MAFB¹.

The t(11;14)(q13;q32) translocation is the most common translocation in MM, where it is seen in approximately 15% of cases^2,3.

Unlike mantle cell lymphoma (MCL), where the breakpoints are clustered in a 1kb region that is 120kb centromeric to the CCND1 gene⁴, the breakpoints in MM cases are dispersed within a 360kb region between CCND1 and MYEOV at 11q13⁵. MYEOV is a putative oncogene, located 360kb centromeric to CCND1, which is thought to be activated in the translocation by becoming closely associated with IGH enhancers. In contrast to IGH rearrangements in other neoplasms, those found in MM have IGH breakpoints predominantly in the C/J region, which, in the case of MYEOV, brings the MYEOV gene under the control of the 3' Eα1 enhancer⁵. In CCND1 translocations by contrast, the Eμ enhancer controls CCND1 expression. MYEOV overexpression is a possible prognostic factor in MM⁶.

The t(11;14)(q13;q32) associated with a favourable outcome in most series and therefore is regarded as neutral with regard to prognosis³.

Fluorescence in situ hybridisation (FISH) microscope image of the CytoCell IGH/MYEOV Plus Translocation, Dual Fusion probe.

What our customers say

Not only do CytoCell offer an extensive range of high-quality FISH probes, the customer support is also excellent — providing fast access to all the probes I need. The probes are highly consistent with bright signals allowing easy scoring of results.

Dr Eric Crawford

Senior Director, Genetics Associates Inc., USA

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Recommended protocol for CytoCell haematology FISH

Select a protocol step to view:

Sample and slide preparation

Spot the cell sample onto a glass microscope slide. Allow to dry.
Immerse the slide in 2x Saline Sodium Citrate (SSC) for 2 minutes at room temperature (RT) without agitation.
Dehydrate in an ethanol series (70%, 85% and 100%), each for 2 minutes at RT.
Allow to dry.