CytoCell ROS1 Breakapart FISH Probe

Product summary

Technology FISH
Application Solid tumour
Areas of interest Glioma, Cholangiocarcinoma (bile duct), Ovarian cancer, Lung cancer
Region 6q22.1
Label
Product Code LPS 022 (10 tests)
LPS 022-S (5 tests)
Regulatory Status In vitro diagnostic. This product is intended to be used on formalin-fixed paraffin-embedded (FFPE) tissues. Disease information supported by the literature and is not a reflection of the intended purpose of this product.

Documentation

Overview

Probe specification

ROS1, 6q22.1, Red
ROS1, 6q22.1, Green

The ROS1 Breakapart probe consists of a green 406kb probe and two red 299kb and 171kb probes, which are positioned on each side of the ROS1 gene.

Probe information

The receptor tyrosine kinase c-ros oncogene 1 (ROS1) is an orphan receptor tyrosine kinase of the insulin receptor family and is located at 6q22¹. ROS1 consists of a large extracellular domain that is composed of six fibronectin repeats, transmembrane domain, and an intracellular kinase domain. While the function of ROS1 is undefined, it has been shown to play an important role in the differentiation of epididymal epithelium².

ROS1 rearrangements have been reported to define a molecular subset of Non Small-Cell Lung Cancer (NSCLC)³. Rearrangements of ROS1 with fusion partners SLC34A2 (solute carrier family 34 (sodium phosphate), member 2)⁴ and CD74 (Cluster of Differentiation 74), have been shown to occur in less than 2% of patients with NSCLC3. Patients harbouring ROS1 rearrangement have been shown to respond to treatment with ALK/MET tyrosine kinase inhibitors, such as Xalkori^® (crizotinib)^5,6.

ROS1 rearrangements with the fusion partner GOPC (golgi-associated PDZ and coiled-coil motif containing) have also been implicated in Glioblastoma and Cholangiocarcinoma^5,7. ROS1 Fusions activate the pSTAT3, PI3K/AKT/mTOR and the SHP-2 phosphatase pathways^8,9.

Fluorescence in situ hybridisation (FISH) microscope image of the CytoCell ROS1 Breakapart probe.

What our customers say

In our hands, CytoCell FISH probes, including the ROS1 Proximal and ROS1 Distal probes, have proven to be of the highest quality with bright, easy to interpret signals, thus providing confidence in our results. OGT's customer support is outstanding, as their staff are extremely knowledgeable and truly care about their customers and their customers’ needs.

Jennie Thurston

Director of Cytogenetics, Carolinas Pathology Group, USA

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References

Matsushime H et al., Mol Cell Biol 1986;6:3000-4
Yeung CH et al., Biol Reprod 1999;61:1062-9
Bergethon K et al., J Clin Oncol 2012;30(8):863-70
Davies KD et al., Cancer Res 2012;72(8):1538-7445
Matsuura et al., Oncol Rep. 2013 Oct;30(4):1675-80
Ou SH et al., Expert Rev Anticancer Ther 2012;12(4):447-56
Yasuda H et al., J Thorac Oncol 2012;7(7):1086-90
Birchmeier C et al., Proc Natl Acad Sci 1987;84:9270-9274
Gu TL et al., PLoS One 2011;6: e15640
Charest A et al., Genes Chromosomes Cancer 2003;37:58-71

FFPE tissue preparation and FISH protocol

Select a protocol step to view:

Heat pretreatment

Heat 50ml Tissue Pretreatment Solution (Reagent 1) in a porcelain wash jar or coplin jar immersed in a waterbath until it is either boiling or 98 - 100°C.
Boil slides for 30 minutes (Note: different incubation times may be required depending on tissue fixation. A 30-minute incubation is a recommended starting point).
Wash in PBS or dH₂O at room temperature (RT) for 2 x 3 minutes.