CytoCell TOP2A Amplification/Deletion FISH Probe

Product summary

Technology FISH
Application Solid tumour
Areas of interest Breast cancer, Ovarian cancer
Region 17q21.2
17p11.1-q11.1
Label
Product Code LPS 002 (10 tests)
LPS 002-S (5 tests)
Regulatory Status In vitro diagnostic. This product is intended to be used on formalin-fixed paraffin-embedded (FFPE) tissues. Disease information supported by the literature and is not a reflection of the intended purpose of this product.

Documentation

Overview

Probe specification

TOP2A, 17q21.2, Red
D17Z1, 17p11.1-q11.1, Green

The TOP2A Amplification/Deletion probe consists of a red 164kb probe spanning the TOP2A gene region. The centromeric probe in green acts as a control for chromosome 17.

Probe information

The Topoisomerase 2A encoding (TOP2A) gene is located on chromosome band 17q21.2, near to the HER2 (ERBB2) oncogene. The TOP2A encoded protein has a function in DNA replication and the transcription of mRNA^1,2. Amplification of the TOP2A gene is frequent in breast cancer and is often accompanied by HER2 amplification³.

TOP2A gene alterations are associated with an increased response to therapy using the anti-cancer drug anthracycline⁴, while patients with tumours that show normal levels of TOP2A and HER2 do not appear to gain any additional benefit from this drug⁴.

Fluorescence in situ hybridisation (FISH) microscope image of the CytoCell TOP2A Amplification/Deletion probe.

What our customers say

The quality and reproducibility of results using the CytoCell kit has been vital in accurately detecting co-deletions in our glioma investigations. We now have a cost-effective test that we can rely on that is also easy to use and interpret. We've been consistently impressed with this kit - not to mention the support offered by OGT's customer service, and have completely transitioned over to CytoCell probes.

Gavin Cuthbert, FRCPath

Head of Cancer Cytogenetics, Northern Genetics Service, Newcastle, UK

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FFPE tissue preparation and FISH protocol

Select a protocol step to view:

Heat pretreatment

Heat 50ml Tissue Pretreatment Solution (Reagent 1) in a porcelain wash jar or coplin jar immersed in a waterbath until it is either boiling or 98 - 100°C.
Boil slides for 30 minutes (Note: different incubation times may be required depending on tissue fixation. A 30-minute incubation is a recommended starting point).
Wash in PBS or dH₂O at room temperature (RT) for 2 x 3 minutes.