Tips include advice on measuring the temperature of washing solutions and why you should rinse your slides in a dehydration series of EtOH washes.
Watch our video on how to prevent stringency issues from affecting your FISH results during post hybridisation washes.
Always balance the pH of wash solutions during preparation, and periodically during use, to ensure they produce optimal results.
Laurence Cambridge
FISH Field Application Specialist, Europe
Try rinsing slides in a dehydration series of EtOH washes (70%, 85%, and 100%) after the room temperature washes to reduce background.
Graham Halford
FISH Field Application Specialist, Europe
When measuring the temperature of washing solutions, make sure to do this inside the jar to replicate the correct conditions.
Jenny Morse
FISH Field Application Scientist, North America
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