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Tips include how to minimize duplication rates in sequencing results.

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After adaptor ligation, use the lowest cycle number possible based on the DNA concentration. This will minimize duplication rates in sequencing results.

Serena Pace Quote Image

Serena Pace

NGS Field Application Scientist, North America

Analysis of amplified prepared library DNA

Figure 1: Analysis of amplified prepared library DNA using an Agilent D1000 ScreenTape assay. The electropherogram shows a single peak in the size range of 200 to 275 bp (+/-10%).

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