Tips include why you should limit pipetting and why small amounts of buffer carryover from washes will not interfere with DNA binding to the beads.
Streptavidin beads are very “sticky”, be sure to limit pipetting.
Huiyan Jin
NGS Field Application Scientist, North America
Do not let the streptavidin beads dry out.
Kicki Bergefall
FAS Manager - Europe
Small amounts of buffer carryover from washes will not interfere with DNA binding to the beads. They will be diluted enough and don’t inhibit subsequent washes.
Serena Pace
NGS Field Application Scientist, North America