CytoCell TLX3 Breakapart FISH Probe

Product summary

Technology FISH
Application Haematology
Areas of interest ALL
Region 5q35.1
Label
Product Code LPH 050 (10 tests)
LPH 050-S (5 tests)
Regulatory Status In vitro diagnostic. This product is intended to be used on Carnoy’s solution (3:1 methanol/acetic acid) fixed haematological samples. Disease information supported by the literature and is not a reflection of the intended purpose of this product.

Documentation

Overview

Probe specification

TLX3, 5q35.1, Red
TLX3, 5q35.1, Green

The TLX3 product consists of a 162kb probe, labelled in red, located centromeric to the TLX3 gene, including the middle part of the RANBP17 gene and the D5S653 marker and a green probe covering a 173kb region located telomeric to the gene, including the NPM1 and FGF18 genes and the RH80894 marker.

Probe information

The TLX3 (T-cell leukemia homeobox 3) gene at 5q35 can be aberrantly expressed in T-cell acute lymphoblastic leukaemia (T- ALL) due to a cryptic translocation¹.

Unlike TLX1, the dysregulation of TLX3 is not brought about by close juxtaposition with T-cell receptor genes, instead, it is brought into contact with another gene that is highly expressed in normal T-cell differentiation: BCL11B at 14q32². The t(5;14)(q35;q32) translocation is cryptic and the breakpoint does not actually disrupt TLX3 but, in the majority of cases, occurs within or downstream of the RANBP17 gene³. RANBP17 is very close to TLX3 and although its expression is not affected by the translocation, TLX3 expression is affected. The t(5;14)(q35;q32) translocation is found in approximately 20% of childhood T-ALL and 13% of adult cases. Rarer TLX3 rearrangements have also been reported: a t(5;7)(q35;q21) translocation involving CDK6 at 7q21 and a t(5;14)(q35;q11) translocation involving TRA/D at 14q11.2¹.

Fluorescence in situ hybridisation (FISH) microscope image of the CytoCell TLX3 Breakapart probe.

What our customers say

The quality of the products we have received from CytoCell have been excellent. The FISH probes they provide to us give intense, strong signals and are a pleasure to count. What has really stood out however has been the level of support and assistance provided by CytoCell’s application specialists. The team worked very closely alongside our own during the adoption of this product and spent many hours with us perfecting the technique, going above and beyond what I would expect during the transition period. Source BioScience absolutely demand high quality products and service to be able to deliver our results with confidence, and that is what we have received from CytoCell.

Neil Ryan

Laboratory Operations Manager, Source BioScience, UK

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Recommended protocol for CytoCell haematology FISH

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Sample and slide preparation

Spot the cell sample onto a glass microscope slide. Allow to dry.
Immerse the slide in 2x Saline Sodium Citrate (SSC) for 2 minutes at room temperature (RT) without agitation.
Dehydrate in an ethanol series (70%, 85% and 100%), each for 2 minutes at RT.
Allow to dry.